Hotspot

rps16-trnQ-CAA

LSC · 6034-6482 · 449 bp

Back to Descurainia

Pi 0.0303

MarkerSeek score 67.90

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 13
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	502-522 bp	1.0	83.4
rps16-trnQ-CAA_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	512-532 bp	1.0	82.6
rps16-trnQ-CAA_p3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	528-548 bp	1.0	81.0
rps16-trnQ-CAA_p4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	529-549 bp	1.0	80.9
rps16-trnQ-CAA_p5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	530-550 bp	1.0	79.2