Hotspot

rpoB

LSC · 19035-22265 · 3231 bp

Back to Deschampsia

Pi 0.000929

MarkerSeek score 55.80

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3231
variable_sites: 3
pi: 0.000929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ACTGTTCATGGGCGAAAAGT	ATGGGCTCTCTATGGATCCC	45.0	55.0	58.0	58.0	205 bp	1.0	80.0
rpoB_p2	ATGGGCGAAAAGTGAGTTGT	ATGGGCTCTCTATGGATCCC	45.0	55.0	58.0	58.0	198 bp	1.0	80.0
rpoB_p3	TGTTCATGGGCGAAAAGTGA	ATGGGCTCTCTATGGATCCC	45.0	55.0	58.0	58.0	203 bp	1.0	79.9
rpoB_p4	TGGGCGAAAAGTGAGTTGTT	ATGGGCTCTCTATGGATCCC	45.0	55.0	58.2	58.0	197 bp	1.0	78.2
rpoB_p5	ATGGGCGAAAAGTGAGTTGT	GCTCTCTATGGATCCCCACA	45.0	55.0	58.0	58.3	194 bp	1.0	78.0