Hotspot

petB

LSC · 70746-72153 · 1408 bp

Back to Deschampsia

Pi 0.00142
MarkerSeek score 59.60
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1408
variable_sites
2
pi
0.00142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TAGTTCGACCGCGGAATTTT TGAAATACCACAACCTACCCT 45.0 42.9 57.9 56.3 1512-1513 bp 1.0 57.5
petB_p2 TAGTTCGACCGCGGAATTTT ACCCATGTTTGTAGCAATGA 45.0 40.0 57.9 55.2 1529-1530 bp 1.0 57.3
petB_p3 ATTATGGTAGTTCGACCGCG TGAAATACCACAACCTACCCT 50.0 42.9 57.9 56.3 1519-1520 bp 1.0 57.1
petB_p4 ATTATGGTAGTTCGACCGCG ACCCATGTTTGTAGCAATGA 50.0 40.0 57.9 55.2 1536-1537 bp 1.0 56.9
petB_p5 TAGTTCGACCGCGGAATTTT ACCCATGTTTGTAGCAATGAA 45.0 38.1 57.9 56.0 1529-1530 bp 1.0 55.8