Hotspot

ndhF

SSC · 101465-103684 · 2220 bp

Back to Deschampsia

Pi 0.0018

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGAGGGTAGGGGGATAGGA	TGGTTTGGTGAATCGGAAACA	55.0	42.9	58.0	58.3	2345 bp	1.0	69.0
ndhF_p2	AAGAGGGTAGGGGGATAGGA	GGTTTGGTGAATCGGAAACA	55.0	45.0	58.0	56.5	2344 bp	1.0	68.1
ndhF_p3	AAGAGGGTAGGGGGATAGGA	TGGTTTGGTGAATCGGAAAC	55.0	45.0	58.0	56.5	2345 bp	1.0	68.1
ndhF_p4	AGAGGGTAGGGGGATAGGAT	TGGTTTGGTGAATCGGAAACA	55.0	42.9	57.8	58.3	2344 bp	1.0	67.9
ndhF_p5	AGAGGGTAGGGGGATAGGAT	GGTTTGGTGAATCGGAAACA	55.0	45.0	57.8	56.5	2343 bp	1.0	67.0