Hotspot

rpl23-accD

LSC · 55854-56279 · 426 bp

Back to Deschampsia

Pi 0.00474

MarkerSeek score 58.80

Primer available yes

Label rpl23-accD

Feature Metadata

label_name: rpl23-accD
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 2
pi: 0.00474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-accD_p1	TCATTACCCTTCAACCAGGT	GCGTGGTAAAGGGGAGAAAA	45.0	50.0	56.0	58.1	559-563 bp	1.0	64.3
rpl23-accD_p2	TCATTACCCTTCAACCAGGTT	GCGTGGTAAAGGGGAGAAAA	42.9	50.0	56.8	58.1	559-563 bp	1.0	62.6
rpl23-accD_p3	TGATCATTACCCTTCAACCAGG	GCGTGGTAAAGGGGAGAAAA	45.5	50.0	57.8	58.1	562-566 bp	1.0	62.2
rpl23-accD_p4	GATCATTACCCTTCAACCAGGT	GCGTGGTAAAGGGGAGAAAA	45.5	50.0	57.8	58.1	561-565 bp	1.0	62.2
rpl23-accD_p5	AGGACCTATTCTGGGACATACA	GCGTGGTAAAGGGGAGAAAA	45.5	50.0	57.7	58.1	600-604 bp	1.0	61.8