Hotspot

trnS-psbD

LSC · 7673-8635 · 963 bp

Back to Deschampsia

Pi 0.00312

MarkerSeek score 58.80

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 3
pi: 0.00312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1108 bp	1.0	78.5
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1095 bp	1.0	77.9
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1079 bp	1.0	77.4
trnS-psbD_p4	GAGCGGAAAGAGAGGGATTC	GCCGGACCATCCTACAAAAA	55.0	50.0	57.8	58.2	1159 bp	1.0	77.0
trnS-psbD_p5	GAGCGGAAAGAGAGGGATTC	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.8	58.2	1146 bp	1.0	76.4