Hotspot

ycf3

LSC · 16474-18454 · 1981 bp

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Pi 0.0122
MarkerSeek score 50.60
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1981
variable_sites
24
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TAATGATGCCCAGAAACCGG TGACATAGGAGTTAGGCGCT 50.0 50.0 57.9 58.2 2904-2924 bp 1.0 79.6
ycf3_p2 TAATGATGCCCAGAAACCGG GCGCTTAACGGACCTTTTTG 50.0 50.0 57.9 58.3 2889-2909 bp 1.0 78.8
ycf3_p3 GCCCAGAAACCGGTTGAATA TGACATAGGAGTTAGGCGCT 50.0 50.0 58.2 58.2 2897-2917 bp 1.0 78.6
ycf3_p4 AAGGTTCGGAAAGAGAGGGA GCGCTTAACGGACCTTTTTG 50.0 50.0 58.0 58.3 2987 bp 0.5 57.2
ycf3_p5 TCGGAAAGAGAGGGATTCGA TGACATAGGAGTTAGGCGCT 50.0 50.0 57.9 58.2 2997 bp 0.5 57.1