Hotspot

rpoC2

LSC · 40578-44732 · 4155 bp

Back to Derris

Pi 0.00818
MarkerSeek score 44.40
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4155
variable_sites
34
pi
0.00818
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 CTTCCCTTCCGGTATCTCGA TGAACTTCCTCGGGAATGAA 55.0 45.0 58.3 56.5 246 bp 1.0 67.2
rpoC2_p2 TCTTCCCTTCCGGTATCTCG TGAACTTCCTCGGGAATGAA 55.0 45.0 58.3 56.5 247 bp 1.0 67.2
rpoC2_p3 TCCCTTCCGGTATCTCGATT TGAACTTCCTCGGGAATGAA 50.0 45.0 57.6 56.5 244 bp 1.0 66.7
rpoC2_p4 TTCCCTTCCGGTATCTCGAT TGAACTTCCTCGGGAATGAA 50.0 45.0 57.6 56.5 245 bp 1.0 66.7
rpoC2_p5 CCCTTCCGGTATCTCGATTG TGAACTTCCTCGGGAATGAA 55.0 45.0 57.6 56.5 243 bp 1.0 66.2