Hotspot

rpoA

LSC · 77554-78555 · 1002 bp

Pi 0.017

MarkerSeek score 54.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1002
variable_sites: 17
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CATTTCGTCGTCCAGTAGCA	TCAAGGGTACACGAAGAGGT	50.0	50.0	58.0	58.0	1618-1667 bp	1.0	81.6
rpoA_p2	AAAACCCATTTCGTCGTCCA	TCAAGGGTACACGAAGAGGT	45.0	50.0	58.0	58.0	1624-1673 bp	1.0	81.4
rpoA_p3	GGTACCATAGTGGCCCTTTG	TCAAGGGTACACGAAGAGGT	55.0	50.0	57.9	58.0	1580-1629 bp	1.0	81.3
rpoA_p4	GTACCATAGTGGCCCTTTGG	TCAAGGGTACACGAAGAGGT	55.0	50.0	57.9	58.0	1579-1628 bp	1.0	81.3
rpoA_p5	TTCCGGTAGTCATTCTGGGT	TCAAGGGTACACGAAGAGGT	50.0	50.0	58.1	58.0	1867-1916 bp	1.0	81.1