Hotspot

trnN-GUU-trnR-ACG

IRa · 128945-129582 · 638 bp

Back to Derris

Pi 0.0173
MarkerSeek score 68.80
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
638
variable_sites
11
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 779 bp 1.0 79.5
trnN-GUU-trnR-ACG_p2 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 820 bp 1.0 78.7
trnN-GUU-trnR-ACG_p3 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 743 bp 1.0 78.4
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 730 bp 1.0 78.3
trnN-GUU-trnR-ACG_p5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 819 bp 1.0 77.9