Hotspot

ndhF-rpl32

SSC · 111839-112552 · 714 bp

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Pi 0.0321
MarkerSeek score 70.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
714
variable_sites
18
pi
0.0321
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAAAAAGT CGATTGCGCTAATGAAAAAGC 40.0 42.9 56.4 57.2 786-925 bp 1.0 58.4
ndhF-rpl32_p2 TTTGTTGCTGTCGGAAAAAGT CGATTGCGCTAATGAAAAAGC 38.1 42.9 57.1 57.2 787-926 bp 1.0 56.4
ndhF-rpl32_p3 TTTTGTTGCTGTCGGAAAAAGT CGATTGCGCTAATGAAAAAGC 36.4 42.9 57.8 57.2 788-927 bp 1.0 53.8
ndhF-rpl32_p4 TTGTTGCTGTCGGAAAAAGT TGTTGTCCAATATCCCCTCT 40.0 45.0 56.4 55.5 759-898 bp 1.0 52.9
ndhF-rpl32_p5 TTGTTGCTGTCGGAAAAAGT CGATTGCGCTAATGAAAAAGCT 40.0 40.9 56.4 58.6 786-925 bp 1.0 52.3