Hotspot

rps12-clpP

LSC · 69299-69508 · 210 bp

Back to Derris

Pi 0.027
MarkerSeek score 58.80
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
210
variable_sites
5
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 ATTGGCTGTCTTGTGTTTCT CCCAAGCCCACGGAATTATT 40.0 50.0 55.5 58.2 270-295 bp 1.0 63.3
rps12-clpP_p2 ATTGGCTGTCTTGTGTTTCT GTCAGCAGTAGAAGCCCAAG 40.0 55.0 55.5 58.3 284-309 bp 1.0 62.9
rps12-clpP_p3 ATTGGCTGTCTTGTGTTTCT TATGCACAAAGAACGGGCAA 40.0 45.0 55.5 58.4 349-374 bp 1.0 61.7
rps12-clpP_p4 ATTGGCTGTCTTGTGTTTCT ATGTCAGCAGTAGAAGCCCA 40.0 50.0 55.5 58.7 286-311 bp 1.0 59.3
rps12-clpP_p5 TGGCTGTCTTGTGTTTCTAA CCCAAGCCCACGGAATTATT 40.0 50.0 54.8 58.2 268-293 bp 1.0 58.2