Hotspot

rps16-trnQ-UUG

LSC · 57154-57620 · 467 bp

Back to Derris

Pi 0.0368
MarkerSeek score 70.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
467
variable_sites
16
pi
0.0368
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 522-547 bp 1.0 85.7
rps16-trnQ-UUG_p2 GTCTTTCAAGTCGCACGTTG CAAATCAAAAGTGGGGCGTG 50.0 50.0 58.0 58.2 581-606 bp 1.0 84.4
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 510-535 bp 1.0 84.3
rps16-trnQ-UUG_p4 GTCTTTCAAGTCGCACGTTG TCAAATCAAAAGTGGGGCGT 50.0 45.0 58.0 58.3 582-607 bp 1.0 83.7
rps16-trnQ-UUG_p5 GCACGTTGCTTTTTACCACA CAAATCAAAAGTGGGGCGTG 45.0 50.0 57.8 58.2 569-594 bp 1.0 82.9