Hotspot

trnG-UCC-psaI

LSC · 52877-53233 · 357 bp

Back to Derris

Pi 0.0393
MarkerSeek score 69.30
Primer available yes
Label trnG-UCC-psaI

Feature Metadata

label_name
trnG-UCC-psaI
feature_type
igs
region
LSC
length_bp
357
variable_sites
14
pi
0.0393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-psaI_p1 ATGAATCAAACGGGGGATCC GGGCCTAATATTTCCGGCAA 50.0 50.0 57.6 58.0 504-505 bp 1.0 79.4
trnG-UCC-psaI_p2 ATGAATCAAACGGGGGATCC ATTTCCGGCAATTGCAATGG 50.0 45.0 57.6 58.0 495-496 bp 1.0 79.3
trnG-UCC-psaI_p3 ATGAATCAAACGGGGGATCC GGCAATTGCAATGGCTTCTT 50.0 45.0 57.6 57.9 489-490 bp 1.0 78.7
trnG-UCC-psaI_p4 ACTATTAAGGGGTCCGTGGA GGGCCTAATATTTCCGGCAA 50.0 50.0 57.4 58.0 480-481 bp 1.0 78.0
trnG-UCC-psaI_p5 ACTATTAAGGGGTCCGTGGA ATTTCCGGCAATTGCAATGG 50.0 45.0 57.4 58.0 471-472 bp 1.0 77.9