Hotspot

trnT-GGU-trnE-UUC

LSC · 29701-30527 · 827 bp

Back to Derris

Pi 0.0249
MarkerSeek score 68.10
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
827
variable_sites
20
pi
0.0249
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT ACTGCCTTTCGTAGTACCCT 50.0 50.0 58.0 58.1 965-974 bp 1.0 83.0
trnT-GGU-trnE-UUC_p2 CCATGGCGTTACTCTACCAC ACTGCCTTTCGTAGTACCCT 55.0 50.0 58.1 58.1 944-953 bp 1.0 82.6
trnT-GGU-trnE-UUC_p3 TGGCGTTACTCTACCACTGA ACTGCCTTTCGTAGTACCCT 50.0 50.0 58.1 58.1 941-950 bp 1.0 82.4
trnT-GGU-trnE-UUC_p4 GAACCGATGACTTACGCCTT TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 959-968 bp 1.0 82.2
trnT-GGU-trnE-UUC_p5 CCGATGACTTACGCCTTACC ACTGCCTTTCGTAGTACCCT 55.0 50.0 58.2 58.1 962-971 bp 1.0 81.8