Hotspot

rbcL-atpB

LSC · 6488-7291 · 804 bp

Back to Derris

Pi 0.0188
MarkerSeek score 70.10
Primer available yes
Label rbcL-atpB

Feature Metadata

label_name
rbcL-atpB
feature_type
igs
region
LSC
length_bp
804
variable_sites
15
pi
0.0188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-atpB_p1 TTAACACCAGCCTTGAACCC CCTGGGGGAAAAGCTACATC 50.0 55.0 58.0 57.9 970-977 bp 1.0 81.1
rbcL-atpB_p2 TTAACACCAGCCTTGAACCC CAAGTGCAGAAACCTCAGGA 50.0 50.0 58.0 57.7 903-910 bp 1.0 79.6
rbcL-atpB_p3 TTAACACCAGCCTTGAACCC TCAAGTGCAGAAACCTCAGG 50.0 50.0 58.0 57.7 904-911 bp 1.0 79.6
rbcL-atpB_p4 TTAACACCAGCCTTGAACCC AAGTGCAGAAACCTCAGGAC 50.0 50.0 58.0 57.7 902-909 bp 1.0 79.6
rbcL-atpB_p5 CCTTGAACCCAACACTTGCT CCTGGGGGAAAAGCTACATC 50.0 55.0 58.6 57.9 960-967 bp 1.0 76.4