Hotspot

trnH-GUG-psbA

LSC · 130-401 · 272 bp

Back to Derris

Pi 0.048
MarkerSeek score 74.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
272
variable_sites
12
pi
0.048
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCCTCTAGATCTAGCTGCGA 55.0 55.0 58.1 57.8 324-339 bp 1.0 84.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCCCTCTAGATCTAGCTGCG 55.0 55.0 58.1 57.8 325-340 bp 1.0 84.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 338-353 bp 1.0 84.2
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCCCTCTAGATCTAGCTGCG 50.0 55.0 57.9 57.8 339-354 bp 1.0 84.2
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 337-352 bp 1.0 84.2