Hotspot

ndhF

Genome · 106085-108328 · 2244 bp

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Pi 0.0402

MarkerSeek score 76.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2244
variable_sites: 166
pi: 0.0402
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAATTGGAGCTTCGTGAGA	CCGCCGATGTCAATTATTGC	50.0	50.0	58.0	58.0	2415-2636 bp	1.0	89.7
ndhF_p2	CGAATTGGAGCTTCGTGAGA	ATCTTCGACTCCCAACCAGA	50.0	50.0	58.0	58.1	2131-2336 bp	1.0	89.4
ndhF_p3	CGAATTGGAGCTTCGTGAGA	GGGGGTGATTTGCAAACCTA	50.0	50.0	58.0	58.1	2328-2533 bp	1.0	89.3
ndhF_p4	CGAATTGGAGCTTCGTGAGA	CGTCGTGAAATCTTCCCTGT	50.0	50.0	58.0	57.9	2453-2674 bp	1.0	89.3
ndhF_p5	CGAATTGGAGCTTCGTGAGA	CGACTCCCAACCAGAAGAAG	50.0	55.0	58.0	57.9	2126-2331 bp	1.0	89.1