Hotspot

trnP-GGG-trnL-UAG

Genome · 110138-110431 · 294 bp

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Pi 0.0913

MarkerSeek score 83.10

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 294
variable_sites: 48
pi: 0.0913
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GATTCGAACCTGTGACCTCC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	397-423 bp	1.0	90.1
trnP-GGG-trnL-UAG_p2	GATTCGAACCTGTGACCTCC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	395-421 bp	1.0	90.1
trnP-GGG-trnL-UAG_p3	GGATTCGAACCTGTGACCTC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	398-424 bp	1.0	90.1
trnP-GGG-trnL-UAG_p4	GGATTCGAACCTGTGACCTC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	396-422 bp	1.0	90.1
trnP-GGG-trnL-UAG_p5	GATTCGAACCTGTGACCTCC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	384-410 bp	1.0	89.3