Hotspot

rpl32-trnP-GGG

Genome · 109370-110063 · 694 bp

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Pi 0.0817

MarkerSeek score 82.90

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 694
variable_sites: 104
pi: 0.0817
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	GCAATAATTGACATCGGCGG	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.0	58.0	1805-1853 bp	1.0	89.0
rpl32-trnP-GGG_p2	GCAATAATTGACATCGGCGG	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1803-1851 bp	1.0	89.0
rpl32-trnP-GGG_p3	GCAATAATTGACATCGGCGG	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	1419-1459 bp	1.0	88.8
rpl32-trnP-GGG_p4	GCAATAATTGACATCGGCGG	GGAGGTCACAGGTTCGAATC	50.0	55.0	58.0	58.0	1420-1460 bp	1.0	88.8
rpl32-trnP-GGG_p5	ACAGGGAAGATTTCACGACG	GAGGTCACAGGTTCGAATCC	50.0	55.0	57.9	58.0	1389-1421 bp	0.75	74.5