Hotspot

infA-rps8

Genome · 76300-76435 · 136 bp

Back to Deparia

Pi 0.0502

MarkerSeek score 76.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 136
variable_sites: 12
pi: 0.0502
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GGAAGCGATTCCGTAACTGT	TTGGCAAGAAGAAAGACCCC	50.0	50.0	58.0	58.0	386-392 bp	1.0	85.7
infA-rps8_p2	CCTTGCGTGAACGACTTTTC	TTCACGAATAACGACGCCAT	50.0	45.0	58.0	58.0	1125-1149 bp	1.0	85.5
infA-rps8_p3	GGAAGCGATTCCGTAACTGT	TTCACGAATAACGACGCCAT	50.0	45.0	58.0	58.0	585-591 bp	1.0	85.4
infA-rps8_p4	CTTGCGTGAACGACTTTTCC	TTCACGAATAACGACGCCAT	50.0	45.0	58.0	58.0	1126-1148 bp	0.75	74.1
infA-rps8_p5	GCGTGAACGACTTTTCCTTG	TTCACGAATAACGACGCCAT	50.0	45.0	58.0	58.0	1126-1145 bp	0.5	61.5