Hotspot

trnF-GAA-ndhJ

Genome · 46488-46715 · 228 bp

Back to Deparia

Pi 0.0541

MarkerSeek score 82.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 228
variable_sites: 23
pi: 0.0541
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGTCGTACCCAACTTCTACG	50.0	55.0	58.1	58.1	294-302 bp	1.0	87.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACTGATGCCTGAAAGTTGGG	50.0	50.0	58.1	58.1	339-347 bp	1.0	86.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CTGATGCCTGAAAGTTGGGT	50.0	50.0	58.1	58.1	338-346 bp	1.0	86.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TCATCCACACCTTAGGCGTA	50.0	50.0	58.1	58.1	360-368 bp	1.0	85.2
trnF-GAA-ndhJ_p5	CCGAGATAGCTCAGTCGGTA	CTGATGCCTGAAAGTTGGGT	55.0	50.0	58.1	58.1	376-384 bp	1.0	84.9