Hotspot

trnT-GGU-trnfM-CAU

Genome · 35463-35961 · 499 bp

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Pi 0.0568

MarkerSeek score 78.30

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 499
variable_sites: 52
pi: 0.0568
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	AGCAATATCACTCAAGCGGG	TGCCCGGAGTATTGAAATCG	50.0	50.0	58.0	58.1	1037-1047 bp	1.0	87.1
trnT-GGU-trnfM-CAU_p2	GCAATATCACTCAAGCGGGA	TGCCCGGAGTATTGAAATCG	50.0	50.0	58.0	58.1	1036-1046 bp	1.0	87.1
trnT-GGU-trnfM-CAU_p3	CGCATTCAATTCCCGAGGTA	TGCCCGGAGTATTGAAATCG	50.0	50.0	58.1	58.1	1140-1150 bp	1.0	87.1
trnT-GGU-trnfM-CAU_p4	AGCAATATCACTCAAGCGGG	CACCTGTTCGTCTCCATAGC	50.0	55.0	58.0	58.1	1148-1158 bp	1.0	86.3
trnT-GGU-trnfM-CAU_p5	CTGGAAATGGTAGGGAGTGC	TGCCCGGAGTATTGAAATCG	55.0	50.0	58.0	58.1	915 bp	0.5	59.8