Hotspot

trnS-UGA-psbC

Genome · 31253-31548 · 296 bp

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Pi 0.0541
MarkerSeek score 78.30
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
Genome
length_bp
296
variable_sites
30
pi
0.0541
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GGGTTCGAATCCCTCTCTCT TCTATGACACCCCTTAATTGA 55.0 38.1 58.2 53.6 328-346 bp 1.0 50.0
trnS-UGA-psbC_p2 GGGTTCGAATCCCTCTCTCT TTCTATGACACCCCTTAATTGA 55.0 36.4 58.2 54.5 329-347 bp 1.0 50.0
trnS-UGA-psbC_p3 GGGTTCGAATCCCTCTCTCT CTTTCTATGACACCCCTTAATTGA 55.0 37.5 58.2 56.4 331-349 bp 1.0 49.9
trnS-UGA-psbC_p4 GGGTTCGAATCCCTCTCTCT TTTCTATGACACCCCTTAATTGA 55.0 34.8 58.2 55.3 330-348 bp 1.0 49.9
trnS-UGA-psbC_p5 GGGTTCGAATCCCTCTCTCT CTTTCTATGACACCCCTTAATTG 55.0 39.1 58.2 55.0 331-349 bp 1.0 49.9