Hotspot

petN-trnC-GCA

Genome · 29224-29722 · 499 bp

Back to Deparia

Pi 0.0407

MarkerSeek score 77.30

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 499
variable_sites: 35
pi: 0.0407
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	CGTTTGTCACAATCCGCTTC	AAGACGGAGGACTGCAAATC	50.0	50.0	58.0	57.9	638-648 bp	1.0	84.4
petN-trnC-GCA_p2	CGTTTGTCACAATCCGCTTC	TAGTCAAGCGGTAAGACGGA	50.0	50.0	58.0	58.2	650-660 bp	1.0	83.7
petN-trnC-GCA_p3	TGTCACAATCCGCTTCTTCC	AAGACGGAGGACTGCAAATC	50.0	50.0	58.2	57.9	634-644 bp	1.0	83.3
petN-trnC-GCA_p4	CGTTTGTCACAATCCGCTTC	CCAGTTCGAATCTGGGTGTC	50.0	55.0	58.0	58.3	609-619 bp	1.0	83.3
petN-trnC-GCA_p5	TGTCACAATCCGCTTCTTCC	TAGTCAAGCGGTAAGACGGA	50.0	50.0	58.2	58.2	646-656 bp	1.0	82.6