Hotspot

clpP

LSC · 69130-71159 · 2030 bp

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Pi 0.00111

MarkerSeek score 46.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2030
variable_sites: 6
pi: 0.00111
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTCGGCAGGGTTAACTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2134-2138 bp	1.0	69.8
clpP_p2	AACTCGGCAGGGTTAACTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2136-2140 bp	1.0	69.8
clpP_p3	CTCGGCAGGGTTAACTTCAA	AGGCTTTGTTACGTTTCCACA	50.0	42.9	57.8	58.4	2135-2139 bp	1.0	68.1
clpP_p4	AACTCGGCAGGGTTAACTTC	AGGCTTTGTTACGTTTCCACA	50.0	42.9	57.8	58.4	2137-2141 bp	1.0	68.1
clpP_p5	CTCGGCAGGGTTAACTTCAA	AGGCTTTGTTACGTTTCCAC	50.0	45.0	57.8	56.6	2135-2139 bp	1.0	67.4