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atpA

LSC · 28358-29884 · 1527 bp

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Pi 0.000691

MarkerSeek score 42.20

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.000691
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ATATTGGCATATTGGGGGCG	TGAATCCGAAACGCTTGAGT	50.0	45.0	58.1	57.8	1701-1702 bp	1.0	77.7
atpA_p2	ATATTGGCATATTGGGGGCG	ATCCGAAACGCTTGAGTAGC	50.0	50.0	58.1	58.4	1698-1699 bp	1.0	76.2
atpA_p3	ATTGGGGGCGATGAAACAAA	TGAATCCGAAACGCTTGAGT	45.0	45.0	58.4	57.8	1691-1692 bp	1.0	76.0
atpA_p4	TTGGGGGCGATGAAACAAAT	TGAATCCGAAACGCTTGAGT	45.0	45.0	58.4	57.8	1690-1691 bp	1.0	76.0
atpA_p5	TGGGGGCGATGAAACAAATA	TGAATCCGAAACGCTTGAGT	45.0	45.0	57.5	57.8	1689-1690 bp	1.0	74.8