Hotspot

psbB-psbT

LSC · 73136-73331 · 196 bp

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Pi 0

MarkerSeek score 32.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGTGCTCGAACCTTGTTTAGA	TGAAATTTTCGGCGGTTCTC	47.6	45.0	58.2	57.0	399-407 bp	1.0	63.6
psbB-psbT_p2	GGTGCTCGAACCTTGTTTAG	TGAAATTTTCGGCGGTTCTC	50.0	45.0	56.7	57.0	399-407 bp	1.0	63.5
psbB-psbT_p3	GGTGCTCGAACCTTGTTTAGA	AGTTGAAATTTTCGGCGGTTC	47.6	42.9	58.2	58.0	402-410 bp	1.0	63.4
psbB-psbT_p4	GGTGCTCGAACCTTGTTTAGA	GTTGAAATTTTCGGCGGTTCT	47.6	42.9	58.2	58.0	401-409 bp	1.0	63.4
psbB-psbT_p5	GGTGCTCGAACCTTGTTTAG	GTTGAAATTTTCGGCGGTTCT	50.0	42.9	56.7	58.0	401-409 bp	1.0	63.3