Hotspot

ndhC-trnV-UAC

LSC · 50372-51145 · 774 bp

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Pi 0.0016

MarkerSeek score 45.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 4
pi: 0.0016
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	908-909 bp	1.0	76.5
ndhC-trnV-UAC_p2	CTCGGAGCTAAAAATCCCGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	907-908 bp	1.0	74.2
ndhC-trnV-UAC_p3	ACTCGGAGCTAAAAATCCCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	907-908 bp	1.0	73.7
ndhC-trnV-UAC_p4	ACTCGGAGCTAAAAATCCCG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	896-897 bp	1.0	72.6
ndhC-trnV-UAC_p5	CCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.9	57.8	917-918 bp	1.0	71.8