Hotspot

atpI-atpH

LSC · 25289-26397 · 1109 bp

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Pi 0.00164

MarkerSeek score 43.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1109
variable_sites: 4
pi: 0.00164
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCAACTTTAGCCGCG	ATAACGGAAGCGGCAGAAAT	45.0	45.0	58.5	58.0	1196-1198 bp	1.0	75.9
atpI-atpH_p2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1252-1254 bp	1.0	75.3
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.5	57.9	1212-1214 bp	1.0	75.0
atpI-atpH_p4	TCAGTGGTTCCTATCCCTGT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.4	58.0	1257-1259 bp	1.0	74.9
atpI-atpH_p5	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	45.0	45.0	58.5	58.2	1197-1199 bp	1.0	74.7