Hotspot

ycf1

SSC · 126585-132236 · 5652 bp

Back to Dendropanax

Pi 0.00797

MarkerSeek score 57.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5652
variable_sites: 64
pi: 0.00797
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCGCGATTTCCTTGGTTT	GGTTCTTCCCAGAATTTGTGC	45.0	47.6	58.2	57.7	198-270 bp	1.0	80.2
ycf1_p2	ATCCGCGATTTCCTTGGTTT	ACCGTGGATTATACCAAGCA	45.0	45.0	58.2	56.6	152-224 bp	1.0	79.6
ycf1_p3	CCGCGATTTCCTTGGTTTTG	GGTTCTTCCCAGAATTTGTGC	50.0	47.6	58.3	57.7	196-268 bp	1.0	79.1
ycf1_p4	CCGCGATTTCCTTGGTTTTG	ACCGTGGATTATACCAAGCA	50.0	45.0	58.3	56.6	150-222 bp	1.0	78.5
ycf1_p5	TCCGCGATTTCCTTGGTTTT	GGTTCTTCCCAGAATTTGTGC	45.0	47.6	58.4	57.7	197-269 bp	1.0	78.5