Hotspot

rpl16

LSC · 83741-85100 · 1360 bp

Back to Dendropanax

Pi 0.00196

MarkerSeek score 47.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1360
variable_sites: 4
pi: 0.00196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGAAAAAGAAATCAGCACCTGC	AAATTCGATTGGCCATTTGA	40.9	35.0	57.8	54.1	1462-1464 bp	1.0	40.2
rpl16_p2	AGAAAAAGAAATCAGCACCTGC	AAAATTCGATTGGCCATTTGA	40.9	33.3	57.8	55.0	1463-1465 bp	1.0	40.2
rpl16_p3	AGAAAAAGAAATCAGCACCTGC	AATTCGATTGGCCATTTGAT	40.9	35.0	57.8	53.9	1461-1463 bp	1.0	40.2
rpl16_p4	AGAAAAAGAAATCAGCACCTGC	AAAAATTCGATTGGCCATTTGA	40.9	31.8	57.8	55.8	1464-1466 bp	1.0	40.2
rpl16_p5	AGAAAAAGAAATCAGCACCTGC	AAATTCGATTGGCCATTTGAT	40.9	33.3	57.8	54.7	1462-1464 bp	1.0	40.2