Hotspot

trnN(GUU)-trnR(ACG)

IRa · 132636-133223 · 588 bp

Back to Dendropanax

Pi 0.00567

MarkerSeek score 49.60

Primer available yes

Label trnN(GUU)-trnR(ACG)

Feature Metadata

label_name: trnN(GUU)-trnR(ACG)
feature_type: igs
region: IRa
length_bp: 588
variable_sites: 5
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-trnR(ACG)_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	770 bp	1.0	78.0
trnN(GUU)-trnR(ACG)_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	693 bp	1.0	77.6
trnN(GUU)-trnR(ACG)_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	680 bp	1.0	77.6
trnN(GUU)-trnR(ACG)_p4	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	807 bp	1.0	77.4
trnN(GUU)-trnR(ACG)_p5	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	769 bp	1.0	77.2