Hotspot

ndhF-rpl32

SSC · 114875-116053 · 1179 bp

Back to Dendropanax

Pi 0.00283

MarkerSeek score 36.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1179
variable_sites: 5
pi: 0.00283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAAGGTATTATCCACGCA	TAAGGCTGCCCAATATCCCT	42.9	50.0	56.8	58.2	1306-1309 bp	1.0	61.4
ndhF-rpl32_p2	AGGAAAGGTATTATCCACGCA	GACAACGCTTTTAAGGCTGC	42.9	50.0	56.8	58.3	1317-1320 bp	1.0	60.3
ndhF-rpl32_p3	AGGAAAGGTATTATCCACGCA	GCTGCCCAATATCCCTTTCT	42.9	50.0	56.8	57.6	1302-1305 bp	1.0	59.8
ndhF-rpl32_p4	AGGAAAGGTATTATCCACGCA	GGCTGCCCAATATCCCTTTC	42.9	55.0	56.8	58.7	1303-1306 bp	1.0	57.4
ndhF-rpl32_p5	AGGAAAGGTATTATCCACGCAT	TAAGGCTGCCCAATATCCCT	40.9	50.0	57.3	58.2	1306-1309 bp	1.0	57.4