Hotspot

trnR(ACG)-trnN(GUU)

IRb · 110145-110732 · 588 bp

Back to Dendropanax

Pi 0.00567

MarkerSeek score 49.60

Primer available yes

Label trnR(ACG)-trnN(GUU)

Feature Metadata

label_name: trnR(ACG)-trnN(GUU)
feature_type: igs
region: IRb
length_bp: 588
variable_sites: 5
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ACG)-trnN(GUU)_p1	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	770 bp	1.0	78.0
trnR(ACG)-trnN(GUU)_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	693 bp	1.0	77.6
trnR(ACG)-trnN(GUU)_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	680 bp	1.0	77.6
trnR(ACG)-trnN(GUU)_p4	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	807 bp	1.0	77.4
trnR(ACG)-trnN(GUU)_p5	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	769 bp	1.0	77.2