Hotspot

ndhC-trnV(UAC)

LSC · 52986-53996 · 1011 bp

Back to Dendropanax

Pi 0.00198

MarkerSeek score 60.00

Primer available yes

Label ndhC-trnV(UAC)

Feature Metadata

label_name: ndhC-trnV(UAC)
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV(UAC)_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1142-1147 bp	1.0	77.5
ndhC-trnV(UAC)_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1154-1159 bp	1.0	75.9
ndhC-trnV(UAC)_p3	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1141-1146 bp	1.0	74.8
ndhC-trnV(UAC)_p4	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	1147-1152 bp	1.0	73.8
ndhC-trnV(UAC)_p5	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1130-1135 bp	1.0	73.6