Hotspot

trnT(UGU)-trnL(UAA)

LSC · 49101-49924 · 824 bp

Back to Dendropanax

Pi 0.00175
MarkerSeek score 60.00
Primer available yes
Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name
trnT(UGU)-trnL(UAA)
feature_type
igs
region
LSC
length_bp
824
variable_sites
2
pi
0.00175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(UGU)-trnL(UAA)_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 803-874 bp 1.0 83.1
trnT(UGU)-trnL(UAA)_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 811-882 bp 1.0 82.6
trnT(UGU)-trnL(UAA)_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 835-906 bp 1.0 82.2
trnT(UGU)-trnL(UAA)_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 843-914 bp 1.0 81.7
trnT(UGU)-trnL(UAA)_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 817-888 bp 1.0 81.2