Hotspot

trnC(GCA)-petN

LSC · 29612-30291 · 680 bp

Back to Dendropanax

Pi 0.00196

MarkerSeek score 61.10

Primer available yes

Label trnC(GCA)-petN

Feature Metadata

label_name: trnC(GCA)-petN
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 2
pi: 0.00196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(GCA)-petN_p1	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	55.0	45.0	57.9	58.0	766-806 bp	1.0	81.4
trnC(GCA)-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	726-766 bp	1.0	81.3
trnC(GCA)-petN_p3	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	55.0	50.0	57.9	57.8	760-800 bp	1.0	79.9
trnC(GCA)-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	720-760 bp	1.0	79.9
trnC(GCA)-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	744-784 bp	1.0	79.1