Hotspot

rps16-trnQ(UUG)

LSC · 6386-8181 · 1796 bp

Back to Dendropanax

Pi 0.00193
MarkerSeek score 64.00
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1796
variable_sites
5
pi
0.00193
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1804-1869 bp 1.0 82.3
rps16-trnQ(UUG)_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1819-1884 bp 1.0 80.4
rps16-trnQ(UUG)_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1818-1883 bp 1.0 80.4
rps16-trnQ(UUG)_p4 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1875-1940 bp 1.0 79.2
rps16-trnQ(UUG)_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1808-1873 bp 1.0 77.5