Hotspot

ndhF

SSC · 108935-111193 · 2259 bp

Back to Dendrocnide

Pi 0.00679

MarkerSeek score 37.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 29
pi: 0.00679
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	TTAGGGAACGCCAAGTCTTG	50.0	50.0	58.0	57.8	2894-2929 bp	1.0	80.1
ndhF_p2	CCGCTGAAGAAGATCCTTCC	TTAGGGAACGCCAAGTCTTG	55.0	50.0	58.1	57.8	2863-2898 bp	1.0	79.8
ndhF_p3	CGCTGAAGAAGATCCTTCCC	TTAGGGAACGCCAAGTCTTG	55.0	50.0	58.1	57.8	2862-2897 bp	1.0	79.8
ndhF_p4	GGATCCGCTGAAGAAGATCC	TTAGGGAACGCCAAGTCTTG	55.0	50.0	57.8	57.8	2867-2902 bp	1.0	79.0
ndhF_p5	GGGATCCGCTGAAGAAGATC	TTAGGGAACGCCAAGTCTTG	55.0	50.0	57.8	57.8	2868-2903 bp	1.0	79.0