Hotspot

ndhA

SSC · 118364-120656 · 2293 bp

Back to Dendrocnide

Pi 0.00502

MarkerSeek score 40.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2293
variable_sites: 21
pi: 0.00502
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGATCCAAGTTGGGAATATAGGA	CGCCTGGTTTTATCAATTTGC	39.1	42.9	56.5	56.7	2424-2445 bp	1.0	41.5
ndhA_p2	TGATCCAAGTTGGGAATATAGGA	CGCCTGGTTTTATCAATTTGCA	39.1	40.9	56.5	58.4	2424-2445 bp	1.0	41.5
ndhA_p3	TTGATCCAAGTTGGGAATATAGGA	CGCCTGGTTTTATCAATTTGC	37.5	42.9	57.2	56.7	2425-2446 bp	1.0	41.5
ndhA_p4	TTGATCCAAGTTGGGAATATAGGA	CGCCTGGTTTTATCAATTTGCA	37.5	40.9	57.2	58.4	2425-2446 bp	1.0	41.5
ndhA_p5	TGATCCAAGTTGGGAATATAGG	CGCCTGGTTTTATCAATTTGC	40.9	42.9	55.0	56.7	2424-2445 bp	1.0	41.5