Hotspot

rps18-rpl20

LSC · 67569-67839 · 271 bp

Back to Dendrocnide

Pi 0.0141

MarkerSeek score 40.80

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 7
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CGCCTTGCTTTCCAAAGAAG	ATGAAATGAGGGAGGAGGCT	50.0	50.0	57.9	58.1	1453-1511 bp	1.0	82.1
rps18-rpl20_p2	CGCCTTGCTTTCCAAAGAAG	TCGTGGTCGGGAAGGTTATA	50.0	50.0	57.9	57.9	1294-1352 bp	1.0	82.1
rps18-rpl20_p3	ACAATCGGGGGATCGAATTG	TCTGTACAAGGAGCAGTTGC	50.0	50.0	58.0	57.8	610-615 bp	1.0	80.6
rps18-rpl20_p4	ACAATCGGGGGATCGAATTG	ATGAAATGAGGGAGGAGGCT	50.0	50.0	58.0	58.1	1173-1178 bp	1.0	80.3
rps18-rpl20_p5	ACAATCGGGGGATCGAATTG	TCGTGGTCGGGAAGGTTATA	50.0	50.0	58.0	57.9	1014-1019 bp	1.0	80.2