Hotspot

atpB-rbcL

LSC · 53543-54323 · 781 bp

Back to Dendrocnide

Pi 0.00363

MarkerSeek score 61.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 5
pi: 0.00363
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGGTGGAAAAGCTACATCCA	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	919-926 bp	1.0	79.0
atpB-rbcL_p2	GGAGCCTCAGGACTAGAACT	GGTGACATAAGTCCCTCCCT	55.0	55.0	57.8	58.1	846-853 bp	1.0	78.7
atpB-rbcL_p3	ATTTGAGCAATACGCCCCAA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.2	58.1	885-892 bp	1.0	78.6
atpB-rbcL_p4	AAGCTACATCCAGTACCGGA	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	911-918 bp	1.0	78.6
atpB-rbcL_p5	CTACATCCAGTACCGGACCA	GGTGACATAAGTCCCTCCCT	55.0	55.0	58.2	58.1	908-915 bp	1.0	78.0