Hotspot

ycf3-trnS-GGA

LSC · 43761-44647 · 887 bp

Back to Dendrocnide

Pi 0.0105

MarkerSeek score 65.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 18
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTCTTTCTCCCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	57.9	58.1	1047-1056 bp	1.0	80.6
ycf3-trnS-GGA_p2	AGCAAATGCCTCTTTCTCCC	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.2	58.1	1056-1065 bp	1.0	80.2
ycf3-trnS-GGA_p3	CTTTCTCCCCCGAAGTTGTC	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.2	58.1	1045-1054 bp	1.0	79.9
ycf3-trnS-GGA_p4	CTCTTTCTCCCCCGAAGTTG	TTCCAATGCTACGCCTTGAA	55.0	45.0	57.9	57.8	1018-1027 bp	1.0	79.9
ycf3-trnS-GGA_p5	AGCAAATGCCTCTTTCTCCC	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.2	57.8	1027-1036 bp	1.0	79.5