Hotspot

psaA-ycf3

LSC · 40973-41702 · 730 bp

Back to Dendrocnide

Pi 0.00479

MarkerSeek score 58.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 6
pi: 0.00479
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGCCCCTTAGCTATTGTTC	AGCGCAGAATTGGTTGAAGA	55.0	45.0	58.0	58.1	823-896 bp	1.0	83.6
psaA-ycf3_p2	GGGCCCCTTAGCTATTGTTC	AAGATCACAAGGCGTTTCGA	55.0	45.0	58.0	57.8	807-880 bp	1.0	83.1
psaA-ycf3_p3	GGGCCCCTTAGCTATTGTTC	AGATCACAAGGCGTTTCGAA	55.0	45.0	58.0	57.8	806-879 bp	1.0	83.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	783-856 bp	1.0	82.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	766-839 bp	1.0	82.4