Hotspot

psbZ-trnG-GCC

LSC · 35091-35566 · 476 bp

Back to Dendrocnide

Pi 0.0091

MarkerSeek score 55.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 8
pi: 0.0091
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGATGGTTGGTTGGGTAAT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.9	58.1	629-651 bp	1.0	80.8
psbZ-trnG-GCC_p2	CCGATGGTTGGTTGGGTAAT	GAATCGAACCCGCATCTTCT	50.0	50.0	57.9	58.1	630-652 bp	1.0	80.8
psbZ-trnG-GCC_p3	CCGATGGTTGGTTGGGTAAT	CATCTTCTCCGTGGCAAAGA	50.0	50.0	57.9	57.9	618-640 bp	1.0	80.5
psbZ-trnG-GCC_p4	CCGATGGTTGGTTGGGTAAT	CTTCTCCGTGGCAAAGAGAA	50.0	50.0	57.9	57.8	615-637 bp	1.0	80.0
psbZ-trnG-GCC_p5	TTTGCTTCTCCCGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	639-661 bp	1.0	78.1