Hotspot

rpoB-trnC-GCA

LSC · 25979-27128 · 1150 bp

Back to Dendrocnide

Pi 0.00594

MarkerSeek score 47.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 13
pi: 0.00594
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTCCAACCCCAAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.7	58.1	1232-1260 bp	1.0	78.3
rpoB-trnC-GCA_p2	CTCATTTCCAACCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1233-1261 bp	1.0	77.9
rpoB-trnC-GCA_p3	TCATTTCCAACCCCAAGCAT	TTGTTTATCAGGCGACACCC	45.0	50.0	57.7	58.2	1250-1278 bp	1.0	77.5
rpoB-trnC-GCA_p4	CTCATTTCCAACCCCAAGCA	TTGTTTATCAGGCGACACCC	50.0	50.0	58.4	58.2	1251-1279 bp	1.0	77.1
rpoB-trnC-GCA_p5	TCATTTCCAACCCCAAGCAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.7	58.4	1214-1242 bp	1.0	76.1