Hotspot

trnS-GCU-trnG-UCC

LSC · 7471-8122 · 652 bp

Back to Dendrocnide

Pi 0.00946
MarkerSeek score 50.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
652
variable_sites
12
pi
0.00946
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGATATTAATCAACCCGGGGG 50.0 52.4 58.0 57.9 766-783 bp 1.0 73.5
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA GGGATATTAATCAACCCGGGG 50.0 52.4 58.0 57.9 767-784 bp 1.0 73.5
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC GGATATTAATCAACCCGGGGG 50.0 52.4 58.0 57.9 765-782 bp 1.0 73.5
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGGATATTAATCAACCCGGGG 50.0 52.4 58.0 57.9 766-783 bp 1.0 73.5
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA GGATATTAATCAACCCGGGGG 50.0 52.4 58.2 57.9 820-837 bp 1.0 72.2