Hotspot

petD

LSC · 75335-76565 · 1231 bp

Back to Dendrocalamus

Pi 0.00204

MarkerSeek score 48.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1231
variable_sites: 22
pi: 0.00204
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGAAAGAAAATAAGGCGGA	CACGGTACTTCACGGTTGAA	40.0	50.0	55.4	58.1	1290-1293 bp	1.0	59.4
petD_p2	ACGAAAGAAAATAAGGCGGA	CCTATGCACGGTACTTCACG	40.0	55.0	55.4	58.4	1296-1299 bp	1.0	57.0
petD_p3	ACGAAAGAAAATAAGGCGGA	CTATGCACGGTACTTCACGG	40.0	55.0	55.4	58.4	1295-1298 bp	1.0	57.0
petD_p4	ACGAAAGAAAATAAGGCGGAT	CACGGTACTTCACGGTTGAA	38.1	50.0	56.0	58.1	1290-1293 bp	1.0	56.0
petD_p5	ACGAAAGAAAATAAGGCGGA	ACCTATGCACGGTACTTCAC	40.0	50.0	55.4	57.3	1297-1300 bp	1.0	55.2