Hotspot

ndhK

LSC · 51075-51815 · 741 bp

Back to Dendrocalamus

Pi 0.00266

MarkerSeek score 47.70

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 741
variable_sites: 19
pi: 0.00266
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.2	58.0	979 bp	1.0	79.5
ndhK_p2	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	45.0	50.0	57.8	58.0	978 bp	1.0	79.5
ndhK_p3	GCATTGTCTTGACCTCTCCC	TCTCTACCCTTGGGCAATGA	55.0	50.0	58.3	58.0	963 bp	1.0	79.3
ndhK_p4	GCTGCATTGTCTTGACCTCT	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.2	58.0	966 bp	0.086957	41.8
ndhK_p5	GCTGCATTGTCTTGACCTCT	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.2	57.8	881 bp	0.086957	40.5